cd15 cells  (Miltenyi Biotec)

Journal: Lipids in Health and Disease

Article Title: Expression of cell-surface activation markers on human CD15 + cells after selected non esterified fatty acid supplementations

doi: 10.1186/s12944-025-02729-w

Figure Lengend Snippet: Flow cytometry analysis of CD15 + cells with ( A ) unstained, ( B ) isotype- PE control and ( C ) CD45 expression

Article Snippet: CD15 positive cells were extracted from whole blood using the autoMACS system (Miltenyi Biotec Ltd, Surrey, UK) with CD15 + microbeads.

Techniques: Flow Cytometry, Control, Expressing

Journal: Lipids in Health and Disease

Article Title: Expression of cell-surface activation markers on human CD15 + cells after selected non esterified fatty acid supplementations

doi: 10.1186/s12944-025-02729-w

Figure Lengend Snippet: FACS Analysis for the expression of CD11a ( A ), CD11b ( B ) and CD62L ( C ) on isolated human CD15 + cells after 3 h of fatty acid (oleate, linoleate, arachidonate and palmitate) supplementation. The comparisons are made by one-way Anova followed by Tukey HSD post-hoc testing, and the p-values presented are only for the comparisons of individual fatty acid supplementation with the controls

Article Snippet: CD15 positive cells were extracted from whole blood using the autoMACS system (Miltenyi Biotec Ltd, Surrey, UK) with CD15 + microbeads.

Techniques: Expressing, Isolation

Journal: Lipids in Health and Disease

Article Title: Expression of cell-surface activation markers on human CD15 + cells after selected non esterified fatty acid supplementations

doi: 10.1186/s12944-025-02729-w

Figure Lengend Snippet: Phosphatidylcholine (PC) molecular composition of CD15 + cells. Black bars- diacyl PC species, grey bars- alkylacyl PC species

Article Snippet: CD15 positive cells were extracted from whole blood using the autoMACS system (Miltenyi Biotec Ltd, Surrey, UK) with CD15 + microbeads.

Techniques:

Journal: Lipids in Health and Disease

Article Title: Expression of cell-surface activation markers on human CD15 + cells after selected non esterified fatty acid supplementations

doi: 10.1186/s12944-025-02729-w

Figure Lengend Snippet: Endogenous phosphatidylcholine composition for CD15 + cells both unlabelled and newly synthesised methyl -D 9 -choline labelled diacyl ( A ) and alkylacyl PC ( B ) composition. The comparisons between methyl -D 9 labelled and unlabelled individual species are made by using student- t- test

Article Snippet: CD15 positive cells were extracted from whole blood using the autoMACS system (Miltenyi Biotec Ltd, Surrey, UK) with CD15 + microbeads.

Techniques:

Journal: Lipids in Health and Disease

Article Title: Expression of cell-surface activation markers on human CD15 + cells after selected non esterified fatty acid supplementations

doi: 10.1186/s12944-025-02729-w

Figure Lengend Snippet: A pictorial overview of molecular phosphatidylcholine distribution of CD15 + cellular methyl -D 9 -choline enrichment after 3-hours incubation with selected NEFAs, no supplement ( A ), palmitic acid ( B ), oleic acid ( C ), linoleic acid ( D ), arachidonic acid ( E ), and total methyl -D 9 incorporation for individual NEFAs compared to the controls using student t- test ( F )

Article Snippet: CD15 positive cells were extracted from whole blood using the autoMACS system (Miltenyi Biotec Ltd, Surrey, UK) with CD15 + microbeads.

Techniques: Incubation

Journal: Lipids in Health and Disease

Article Title: Expression of cell-surface activation markers on human CD15 + cells after selected non esterified fatty acid supplementations

doi: 10.1186/s12944-025-02729-w

Figure Lengend Snippet: A pictorial overview of the changes in the methyl -D 9 -choline enrichment individual molecular fractional phosphatidylcholine distribution of diacyl ( A ) and alkylacyl ( B ) in the CD15 + cells after NEFAs supplementation

Article Snippet: CD15 positive cells were extracted from whole blood using the autoMACS system (Miltenyi Biotec Ltd, Surrey, UK) with CD15 + microbeads.

Techniques: